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Shown below is a cloning vector 'Z' that Kamla wants to use to create a recombinant vector with her gene of interest.

The vector consists of sites for three restriction enzymes - ScaI, HindIII and EcoRI. Restriction sites for the same enzymes are also present in the gene of interest. There are two 'ori' sequences - one allows it to replicate in Escherichia coli and another allows replication in Zymomonas mobilis. Apart from this, the vector consists of two antibiotic resistance genes - one against tetracycline (tet) and another against chloramphenicol (cml). 

(a) What is the advantage of having two 'ori' sequences in the same vector? Give a situation in which this would be particularly useful.

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(a) It helps the vector replicate in different hosts.

When adding bacterial genes of interest into plant vectors, having ori's that help the same plasmid replicate in both bacteria and plants would help simplify the process of gene cloning.
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